FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_NAC_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_NAC_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031495
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG21480.10573493904735183TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG6750.030.59207743
ATCGGGG7450.030.53639842
CGGGGGG6050.024.87561244
CGGGGGT1851.4551915E-1124.5941844
AATCGGG9450.024.44403341
CGGACTG2400.023.3363866
GAATCGG10800.021.06445940
AGAATCG13850.016.6784239
GGACTGT3850.016.363367
TCCGGAG17250.015.62292534
CCGGAGA17700.015.02799535
CGGAGAA18850.014.48251436
CTCCGGA19650.014.0710133
GGAGAAT19900.013.894237537
ACTCCGG20750.013.32507532
CCCGGTA1950.002301616612.5638926
CACTCCG23250.012.04280731
GAGAATC21200.011.88659238
TCGGACT3054.0627583E-511.4769115
TCAGGGG2150.004779225711.39515742
Produced by FastQC (version 0.11.5)