FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_MYC2_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_MYC2_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700770
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5200.030.28809448
GTATGCC6300.027.7774447
TGCCGTC6000.026.83300650
CGTATGC6500.025.84583946
ATGCCGT6100.025.24559449
GCCGTCT6300.021.66640351
CGGACTG2101.9663275E-920.0016086
CTCGTAT8500.018.94094744
TATCTCG10250.018.438841
TCGTATG10150.017.24116945
CTGCTTG6150.017.07296459
CCGTCGT2702.417437E-916.85164652
TCTCGTA10800.016.52757643
CCGTCTT8250.016.12101652
CGCTCAT11750.016.08491134
CCGCTCA11750.015.78704433
ATCTCGT13450.014.05187542
TGGGGGG3001.5935075E-714.0052741
CGTCTTC9300.013.924561553
ACCGCTC13500.013.74057432
Produced by FastQC (version 0.11.5)