FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_LBD10_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_LBD10_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461246
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT11620.2519263039679477TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2450.048.5711348
GTATGCC2450.047.14256747
TGCCGTC2350.044.68057350
ATGCCGT2600.044.42280249
GCCGTCT2000.043.7497351
CGTATGC2700.042.7775146
TCGTATG3150.035.55533645
CTCGTAT3150.032.22202344
CCGTCTT2650.031.69791652
TATCTCG3600.029.16648741
CTGCTTG2600.028.26905659
TCTCGTA3600.028.1942743
CTTCTGC2650.027.73567856
ATCTCGT3900.026.9229142
GCTTGAA3500.024.99984661
CGTCTTC3250.024.76907753
CCCGTCG750.001831440523.3331968
CGCTCAT5500.022.90894934
TGCTTGA3400.022.6469260
CCGCTCA5850.022.13661633
Produced by FastQC (version 0.11.5)