FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_HDL_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_HDL_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786183
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT39300.22002224856019792TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7050.043.68726348
GCCGTCT6300.042.7771151
CGTATGC7950.040.0622746
ATGCCGT7200.039.8604949
TGCCGTC7050.039.2192550
GTATGCC8200.038.84085547
CTCGTAT9450.034.81427444
TCGTATG9750.033.02512745
CCGTCTT8950.030.89337252
TCTCGTA12000.027.70790143
TATCTCG13250.026.6788341
CTGCTTG8350.026.40677559
AGGGGGG2800.023.7556151
CGTCTTC11250.023.02186453
ATCTCGT15450.022.879942
CTGCGTG1854.0381565E-1022.70234959
CTTCTGC11750.020.5528756
CGCTCAT18700.019.46493734
CCGCTCA20250.018.14786533
CGGACTG1953.9464248E-717.9514546
Produced by FastQC (version 0.11.5)