FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_HB26_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_HB26_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13642116
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC12650.022.41076350
GCCGTCT12350.019.27099251
TATGCCG15100.019.2381548
CGCGCAC4050.017.28371615
GGCTACA85150.016.64686668
GCTACAC86600.016.48938269
TGGCTAC87700.015.92338667
ATGCCGT17700.015.81899449
CTACACC91400.015.81488370
GTATGCC19600.015.17836547
GCGCGTC4650.014.3008813
GCCCGGA6350.013.7793457
CGATCTT143550.013.56073951
ACGGACT25100.013.38627259
CGTATGC23250.013.24713146
CGCCCGG6250.012.87982556
CGCCCCG4355.7480065E-1012.87338718
TTATCAC106850.012.6808461
TTATCGC40200.012.454371
TTATCAA270800.012.0756361
Produced by FastQC (version 0.11.5)