FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_HALO-bZIP63_SBP-bZIP53_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_HALO-bZIP63_SBP-bZIP53_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16989911
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21600.011.66655148
GTATGCC27050.010.09232147
CGGGGTG9750.010.05118353
TCGTATC67850.09.69776738
ATTCGTA78250.09.1245136
CACGAAT82550.08.86120831
CGTATCG29600.08.74991339
TTCGTAT82750.08.41683537
GTCACGA87450.08.364696529
GAATTCG87750.08.29621434
TATCGCG16650.08.19811641
CGAATTC92550.07.979391633
ATGCCGT26800.07.705147749
TCACGAA97000.07.61332730
AATTCGT100050.07.556146635
AGTCACG95300.07.4553328
CGTATGC31400.07.35661546
CGTATCT53300.07.28885839
TGCCGTC29700.06.952792650
ACGAATT113500.06.722432
Produced by FastQC (version 0.11.5)