FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_HALO-CCA1_SBP-LHY1_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_HALO-CCA1_SBP-LHY1_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8156005
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT102470.12563749041350514TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21800.037.8892948
CGTATGC21650.035.4035846
TGCCGTC18050.035.29028350
GTATGCC24200.034.5655547
ATGCCGT19950.033.3327949
GCCGTCT15150.030.26353551
TCGTATC70950.028.16726538
CGTATCT47700.027.66202439
TATCGCG15350.027.36111841
TGCCGGC8450.026.50844450
ATGCCGG10200.025.39174349
TATCTCG41450.025.07841
CTCGTAT31100.024.64590344
CGTATCG28600.024.23037339
TCGTATG32100.023.1148945
GTATCGC19200.022.42150940
TTCGTAT96100.022.25249937
CGGGGTG6700.021.93994153
ATTCGTA100550.021.92903136
GTATCTC50600.021.51150740
Produced by FastQC (version 0.11.5)