FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_DUO3_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_DUO3_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005886
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG16630.16532688594930242TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG4350.039.42495342
TCGGGGG4100.038.41430743
CGGGGGG3700.035.94563744
AATCGGG5850.033.50398641
CGGGGGT1154.0017767E-1133.47797444
GAATCGG7000.027.9997640
AGAATCG8550.022.9237839
TCCGGAG11350.020.66061634
ACTCCGG11600.020.51706732
CGGAGAA11150.020.08951636
CTCCGGA11700.020.04256433
GGAGAAT11150.019.77561637
CCGGAGA11700.019.74342235
CACTCCG12750.018.39231
GAGAATC11700.017.94856538
TTGCCCC1300.002290661416.15370863
TCACTCC14850.016.026830
GGGGGGA2009.961883E-615.749866562
GTCACTC15950.015.14093829
AGGGGGG1800.001255858513.6157321
Produced by FastQC (version 0.11.5)