FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_DUO1_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_DUO1_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6576996
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTGTAGCCAAAGTCC107180.16296193581385787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGATC46650.029.8602289
CCGCAAC125500.029.119763
CGCAACC97400.029.0018564
ACCGCAA151750.028.7191562
CAACCCG45500.028.5412966
AGCGCTA5350.028.1304359
GCAACCC52500.027.6694165
AACCGCA205200.027.5900731
ACCCGAT49050.026.4012438
GCCTAAG102200.025.71880122
GTAGTGT87350.025.64358528
AGGCGTA39550.025.39785620
GATCTTA101650.025.37592112
GACTCAT126300.025.300562
CCTAAGT101500.025.2410123
ATGGACT100550.024.8877569
AGTAGTG91300.024.6108127
AGCCTAA107550.024.6021521
CGATCTT69900.024.4846211
AAGCCTA107550.024.47198120
Produced by FastQC (version 0.11.5)