FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_DAZ3_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_DAZ3_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4868482
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG7250.026.06861142
CGGGGGG5800.019.31008344
CACTCCG13500.019.18492731
AATCGGG10950.018.85819241
TCGGGGG9350.018.34199743
ACTCCGG14450.016.95478832
TCCGGAG17350.014.927752534
CTCCGGA18100.014.50256733
GAATCGG17300.012.34087440
CAGTCAC22200.011.9818227
CCGGAGA22850.011.64098135
CGGGGGT5156.657501E-1011.55324244
CCAGTCA24550.010.54975626
TCCAGTC26850.010.29781225
AGTCACT25900.010.27013228
AGAATCG21800.010.11454339
CGGAGAA27150.09.7972936
TCACTCC30150.09.40285930
ACTCCAG32900.08.51052423
GAACTCC37150.08.00796821
Produced by FastQC (version 0.11.5)