FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_DAZ2_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_DAZ2_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9911500
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG22300.032.17443542
TCGGGGG22900.027.96903843
CGGGGGG19250.025.63600344
AATCGGG30600.024.24802641
CGGGGGT10300.019.02885844
GAATCGG44500.017.5390840
TCCGGAG63200.014.73080634
ACTCCGG67250.013.63549632
AGAATCG59200.013.59778139
CTCCGGA68250.013.58955233
CCGGAGA70200.013.41149235
CACTCCG71450.013.02990731
CGGAGAA71950.012.842072536
GGAGAAT82700.011.00347337
TCACTCC97600.09.78982230
GAGAATC87300.09.66194938
GGGGGTG19950.09.29811545
GTCACTC112250.08.6680329
AGGGGGG13400.08.3613821
CAGTCAC118050.08.12356227
Produced by FastQC (version 0.11.5)