FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_DAZ1_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_DAZ1_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8845767
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG12350.018.42084542
CGGGGGG9200.017.49980444
TCGGGGG13450.014.05188643
AATCGGG18750.013.25318341
CACTCCG33250.09.89462631
GAATCGG27650.09.49356440
TCCGGAG35750.09.39849534
ACTCCGG35050.09.28662832
CTCCGGA37500.08.95989933
CCGGAGA42950.07.741472235
AGAATCG37200.07.432711639
CGGAGAA49150.07.12097836
GGAGAAT59550.06.171214637
CAGTCAC59650.06.160868627
GCGATCG7150.00430173055.87405937
CCAGTCA62150.05.518843726
TCACTCC65350.05.4628330
AGTCACT65700.05.380456428
AGGGGGG14401.6657377E-65.34903671
TCCAGTC67200.05.26035725