FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_ColSPL8_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_ColSPL8_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850373
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3000.050.1745076
ACTGTAG3050.043.6056759
GGACTGT3700.038.7830057
CCGGACT1102.3646862E-1135.005475
GCGGACT1300.035.0054665
CGGACTA2050.034.1516766
TCGGACT2500.033.605255
TAGTCCG1900.033.15722711
GACTGTA4050.032.8388448
CGCTTGC552.993159E-431.81754163
CAGTCCG2600.028.26866127
CTGTAGA5350.027.47608210
CTACAGT2950.026.10116824
AACGGAC1358.069037E-925.9299774
CTAGTCC2300.025.86904310
GCGCGAG1101.0165622E-625.45403330
GTAGAAG6150.025.04014612
CTAACGC700.001226436224.99949664
CGAGCGG700.001226436224.99949623
ACAGTCC2950.024.91475126
Produced by FastQC (version 0.11.5)