FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_ColSPL16_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_ColSPL16_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564641
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG7600.052.5059476
GACTGTA8100.045.8018048
GGACTGT8950.044.18937
ACTGTAG8900.041.6847849
CAGTCCG5700.039.29767227
GCGGACT3750.037.337565
CTGTAGA10100.036.0390810
CGGACTA3600.035.9762956
TCGGACT3250.034.465445
TAGTCCG3150.034.44394311
AACGGAC3100.033.8748054
TATGCCG4900.033.57094248
CCGGACT3750.032.6703645
ATGCCGT4800.032.08286349
CGCGGAC1251.05501385E-1030.8034884
GTAGAAG12200.030.40939112
GCCGTCT4250.029.64662651
ACAGTCC7700.029.54502126
TGCCGTC5250.029.33290550
GGACTAG4200.029.1662437
Produced by FastQC (version 0.11.5)