FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_ColSPL14_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_ColSPL14_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8940913
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15000.019.8330948
GCCGTCT14400.017.74283651
TGCCGTC16500.017.39372450
GTATGCC18150.016.58381747
CGTATGC21550.014.12975546
CCCTGCG9600.013.48941653
CTAACGC10050.012.88541364
CTAGGCG12800.012.85140453
ATGCCGT25550.011.91766149
TAGGCGG14400.011.90957554
GTCCCCC6159.094947E-1211.38197269
CCCGGGC12000.011.3748633
CAGCCTA15500.011.29018349
GGGCGTC11050.011.085834536
CCGGGCG12050.011.03720834
AGCGCGA15600.010.99345329
CCCCGGG11900.010.88221732
CGGGCGT12250.010.85700935
GCTATCT27550.010.54433439
ACGCAAT15900.010.12566167
Produced by FastQC (version 0.11.5)