FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_ColSPL13_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_ColSPL13_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5416201
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT68470.1264170218202759TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13750.045.053948
TGCCGTC13750.044.03573650
GCCGTCT13550.040.55292551
CGTATGC17050.036.12851746
GTATGCC17800.034.80287647
CGGACTG7150.032.800676
ATGCCGT19200.031.3537249
CCGTCTT19350.028.7592852
CTCGTAT22100.026.44758644
TCGTATG24450.024.9076245
TATCTCG24050.023.86660441
GCTATCT26250.022.13301839
AGCTATC27950.021.91381838
TCTCGTA26200.021.24015643
CTGCTTG21150.021.1817359
ATCTCGT28150.020.7634742
GTCACCT37200.018.44059829
CACCTGA37750.018.17192531
GCGGACT3101.8189894E-1218.0664275
TCACCTG38000.017.9602730
Produced by FastQC (version 0.11.5)