FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_ColSPL11_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_ColSPL11_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3237096
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT34600.10688592491541803TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG9850.046.909236
GGACTGT11550.038.484157
GCCGTCT6850.036.2767251
TGCCGTC7650.036.1431450
ATGCCGT7750.035.67677349
GACTGTA12000.034.9993678
TATGCCG8450.034.3780848
ACTGTAG13300.031.5783779
GCGGACT4000.029.75365
TCGGACT7700.029.0944275
CGGACTA5300.029.0601186
CGTATGC10150.028.96499446
CAGTCCG9200.028.53209127
GTATGCC10650.027.27650347
CCGGACT5550.025.2282755
CTGTAGA16850.025.13307810
ACAGTCC10800.024.30511526
TAGTCCG4900.024.28527511
AACGGAC5350.024.2085344
CTCGTAT12550.023.4258744
Produced by FastQC (version 0.11.5)