FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_ColSPL10_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_ColSPL10_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3358576
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT47650.14187560442282682TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9900.039.24176448
ATGCCGT9850.037.30901349
GCCGTCT8400.037.08271451
TGCCGTC9650.036.26881850
GTATGCC11650.034.24835247
CGTATGC11750.033.3611446
CGGACTG3900.032.3109976
CTCGTAT14450.027.1275744
CCGTCTT12450.025.8630252
TCGTATG16100.024.99957845
CAGTCCG3600.023.33294327
TATCTCG16750.023.1936441
GCTATCT17500.022.39962439
GCGGACT3100.021.4538085
TCTCGTA17500.021.39964143
AGCTATC19250.020.5451138
CTGCTTG12750.020.31338559
ATCTCGT20400.019.38692942
CGTCTTC16100.018.4779553
GGACTGT6750.018.1478427
Produced by FastQC (version 0.11.5)