FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_C24SPL16_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_C24SPL16_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932022
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGT4650.055.6983267
CGGACTG4850.055.572386
GACTGTA4950.049.4944238
ACTGTAG5100.048.7249649
TCGGACT2300.048.7003565
CGGACTA1450.045.8664976
CGCGGAC503.477866E-642.004064
CCGGACT2550.039.8077665
TAGTCCG1700.037.05842611
CAGTCCG3700.036.891527
CTGTAGA6750.036.8144210
GCGGACT1850.035.9494175
GTGCGGG400.002419326234.99962618
ACAGTCC4250.033.76434326
CGGACTC1350.033.706966
TACAGTC4300.033.3717425
TGCCGTC1400.032.4996550
CTACAGT4550.031.53812224
CGTATGC2150.029.30201346
TATGCCG2200.028.63605548
Produced by FastQC (version 0.11.5)