FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_C24SPL15m2_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_C24SPL15m2_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23475835
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27600.012.93461148
GTCACGA81550.010.90114329
TCGTATC74650.010.68974538
TGCCGTC26200.010.55329450
AGTCACG85400.010.204782528
GTATGCC36750.010.09510447
CACGAAT99550.08.78943631
TATCGCG18950.08.68062341
GAATTCG101750.08.59939434
CGAATTC101650.08.57342233
ATGCCGT35750.08.51736949
GCCGTCT23350.08.39389351
CCAGTCA110000.08.30443526
CGTATGC42800.08.17746246
CGTATCT75900.08.06972139
CAGTCAC109300.08.03740527
AGGGGGG28700.07.68560741
TCACGAA125400.07.45205430
TCCAGTC118650.07.404031325
TTCGTAT137700.06.684733437
Produced by FastQC (version 0.11.5)