FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_C24SPL14_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_C24SPL14_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121308
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4300.051.2831846
GGACTGT4400.046.1359567
GACTGTA4850.041.133658
ACTGTAG5500.036.908769
CAGTCCG3550.036.4785527
CTGTAGA5900.033.8132610
ACAGTCC3900.033.20483426
TCGGACT2650.031.7006575
TAGTCCG1352.6011548E-1028.51826311
AGGGGCC500.00721061127.9997517
AGCACGG500.00721061127.9997538
GCGGACT1800.027.2244075
TACAGTC4800.026.97892825
CTACAGT5100.026.76446724
TGCCGTC1101.016946E-625.4543250
CCGGACT2650.025.0963545
GTAGAAG8400.023.74978812
TGCGGAC901.9277832E-423.3352074
AGTCCGA2850.023.33312628
TCTACAG5850.023.33312423
Produced by FastQC (version 0.11.5)