FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_C24SPL11_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_C24SPL11_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5218725
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7350.027.14254850
CGGACTG6150.026.7499686
TATGCCG8800.026.24969948
ATGCCGT7600.023.94709449
CGTATGC9800.023.2140246
GTATGCC11300.021.99089847
GCCGTCT6250.021.8397551
TCGTATC28350.021.72814638
CGTATCT20700.020.79686239
TATCTCG18750.019.22644641
CCGGCGT2952.3646862E-1117.79640652
CGTATCG11200.017.499839
CAGTCCG5200.017.499827
ATGCCGG4750.016.94717449
CTCGTAT13850.016.93121344
TGCCGGC5100.016.47039850
TATCGCG6650.016.31560141
ATTCGTA43100.015.9162936
CTGCGTG3753.6379788E-1215.86648459
CGGCGTC2503.123132E-715.39982253
Produced by FastQC (version 0.11.5)