FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_Al_SPL9_col_B_l1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_Al_SPL9_col_B_l1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976584
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGA2305.717864E-412.17377360
TATGCCG2600.00159650810.76910648
GGCATAC2350.0092571610.42541239
TCTGGGG2700.002185749110.37025123
GTATGCC3600.00235618448.74989947
ACACGGG4050.0068145097.77768853
CATTCAG17750.07.098509333
TCCAGTC26150.06.55824270
GAGTTGC6150.00589543636.260090465
CTCCAGT29300.06.092079669
GTCCTGG10407.774723E-66.0597681
CAGTCAC22350.05.95071466
CACATTC22400.05.937431331
CAGAAAT22150.05.846433637
ATTCAGA23500.05.808443534
GTCACAT21850.05.766523429
TCAGAAA24050.05.530081336
CTGAACT39600.05.47973570
AGTCACA24751.8189894E-125.37367628
CCAGTCA23457.2759576E-125.37307226
Produced by FastQC (version 0.11.5)