FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_AGL18_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_AGL18_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134276
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG708.689076E-735.0055126
CGTATGC1455.9480953E-1026.55122446
TGCCGTC1451.729677E-824.13747650
GCCGTCT1502.4789188E-823.33289351
CGACCCC1652.6630005E-923.33289168
GTATGCC1652.6630005E-923.33289147
CCGGACT800.002664688721.8784455
TGTCGAC2800.021.24959865
ATGCCGT1656.8010195E-821.2117249
TATGCCG1859.969881E-920.8104248
GTCGACC1951.8264473E-819.74321666
TCGACCC2152.6320777E-919.53451367
GACCCCA2053.2421667E-818.78013469
GGACTGT1551.9848885E-518.0641757
CTCACGG1350.00294694515.55526166
GTCGACT1450.004740446314.48248666
AGGGAGG1951.4145485E-414.35870366
GGGAGGT2253.0335568E-513.99973667
TATCTCG3001.598437E-713.99973641
TCGTATG2759.1614857E-713.99973645
Produced by FastQC (version 0.11.5)