FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_AGL104_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_AGL104_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997073
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT19160.19216245951901215TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG1250.042.003766
TGCCGTC4450.041.68494450
TATGCCG4700.040.95700548
GCCGTCT4150.040.48149551
GTATGCC4800.040.10373747
CGTATGC5000.039.19957746
ATGCCGT4750.039.0522149
TCGTATG6200.031.61256245
CCGTCTT5450.031.4675552
GGACTGT1900.031.3154547
CTCGTAT6700.029.25341844
TCTCGTA7100.027.6053443
TATCTCG7750.027.54809241
CTGCGTG1305.4096745E-926.92278759
ATCTCGT8400.026.24971842
CGTCTTC6350.025.90523353
CTGCTTG5350.025.51374459
CGCTCAT10700.024.20534734
CTTCTGC6100.024.09810356
CGATAGG750.001832975823.33308455
Produced by FastQC (version 0.11.5)