FastQCFastQC Report
Wed 5 Oct 2022
H2CMNAFX5_n01_Oct_sp7797hop1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CMNAFX5_n01_Oct_sp7797hop1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7569864
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC527930.6974101516222748TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC238120.31456311500444395TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTGG123940.16372817265937673TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGC119100.15733439861006748TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTGG96960.1280868454175663TruSeq Adapter, Index 3 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)