FastQCFastQC Report
Fri 30 Aug 2024
H2CMKBGXW_n01_HEK19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CMKBGXW_n01_HEK19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16161348
Sequences flagged as poor quality0
Sequence length36
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACC8326115.151866044837349No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACC7231774.474731934489623No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACC3518662.1772070003071526No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACC395900.24496718961809372No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)