FastQCFastQC Report
Fri 30 Aug 2024
H2CMKBGXW_n01_HEK18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CMKBGXW_n01_HEK18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15721962
Sequences flagged as poor quality0
Sequence length36
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACG4167032.6504516421042106No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACG917540.583604005657818No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACG691130.4395952617109748No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACG273200.17376966055508847No Hit
AGGGAAAGCGAAAGTCCCGGAAAGGAGCTGACAGGT157660.10028010498944089No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)