FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670786
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120560.1807283279661497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC158850.027.3636455
TCGCCGT167850.027.2934152
CGCCGTA169950.027.1826853
GTCGCCG181300.027.08316251
CGTATCA162100.026.9445656
GGTCGCC183250.026.85225750
TGGTCGC180450.026.55131549
GCCGTAT183800.026.39108354
CTCGGTG162500.025.69369544
GTGGTCG190500.025.53637948
TCTCGGT164150.025.37146643
ATCTCGG166000.025.34170742
GATCTCG158650.025.23628641
GGTGGTC196050.024.74206247
AGATCTC167600.024.24363340
TCGGTGG196600.023.96078545
CAGATCG38650.023.6385965
AAAAGGG131950.023.632470
CGAGATC21950.023.4430145
GTATCAT176650.023.4176557
Produced by FastQC (version 0.11.5)