FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9474629
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128260.13537205520131712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG20450.023.1549111
CGAGCAG19450.022.7223681
GCGAGCG17350.021.98688511
TGTCGGC12000.020.99826230
CGAGCGT18400.020.35179112
CGCGAAA15200.020.0760521
GGCGAGC19150.019.92033610
CGCGCAG4800.019.7299141
CGCGGAT6350.018.7805291
CGGCGGC13700.018.64809233
CGGGAAG39500.018.4700851
CGCCAAT18750.017.9586071
CGCGAAG11750.017.9108431
CGGCTAG5150.017.707971
CGGCAAT21900.017.4576191
GGGCGAG22100.017.419659
CGCGGAG7050.017.413321
CGCCAAG16750.017.3806481
GGATCCG21800.017.33801537
CGCAAAG25600.016.9896471
Produced by FastQC (version 0.11.5)