FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6420848
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109870.1711144696152284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGCG13650.027.94716611
GGCGAGC14400.026.49158710
CGAGCGT14800.025.77559512
CGCGGAG4750.025.0991291
CGCGGAT3100.023.753681
TGTCGGC11150.023.22704930
GGGCGAG17000.023.0580839
CGGCAAC8900.022.851321
CGCCCAG3550.022.718141
CGGGCAG4500.022.5974161
GGATCCG16800.022.29008137
GAGCGTT17750.022.0832813
CGAGCAG12700.021.5359571
CGGCGGC12050.021.49224933
CCGGTCG3100.021.4745032
CGGGAAG30800.021.2894381
CGGCAAG11400.020.9159411
CGCGCAG1859.778887E-920.8494321
CGCGAAG6800.020.6264441
CGGCCAG2051.4260877E-920.5258271
Produced by FastQC (version 0.11.5)