FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7344079
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99360.1352926622929846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG14800.023.9346221
TGTCGGC18900.023.14636230
CGGCGGC20600.021.91578333
CGCGAAA10900.021.88011
CGGGAAG46700.021.103591
CGCGAAG8900.020.8858781
GCGAGCG12600.020.55410811
GTCGGCG22350.019.88660831
TCGGCGG22750.019.84462232
GGCGAGC13700.018.90390610
CGCCAAA21350.018.8915181
CGAGCGT14450.018.1648112
CGAGCAG14550.018.0786151
CGGTCAG6250.017.9571271
CGCCAAG14150.017.8460831
TGCGGTG11800.017.833481
CGGGCAA7400.017.5362571
CGGGCTA4250.017.3299471
AGTCCGC23150.017.23420712
CGGGCAT4350.016.9315571
Produced by FastQC (version 0.11.5)