FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6824642
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112100.1642577002573908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG11100.024.6412871
CGGCAAG21200.023.9841271
CGAGCAG21300.022.8837381
CGCCAAG17000.021.246141
CGCGAAA15300.020.1689361
CGACAAG20900.020.1338441
CGGGCAG7550.019.5071621
CGGGAAG33500.019.15571
CGGCAAT19150.018.6776891
TGCGGTG12400.018.3816051
CGCCAAA27200.018.306751
CGCGGAG6350.018.2235071
CGGGCTG3550.017.780171
CGGCAAC15350.017.1334441
CGCCAAT17000.016.9144021
CGCGCAG3750.016.8318961
CGGCAAA46950.016.506251
CGGGAAA27950.016.4354361
AGCGGAG19050.016.382751
GCGGTAG24450.016.3388462
Produced by FastQC (version 0.11.5)