FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9726109
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151970.15624953411482434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG7500.026.1793981
CGGCAAG26250.025.244421
CGCGAAA19550.023.6733681
CGCGAAG13850.023.5432321
CGGGCAG11950.022.2986531
CGAGCAG23100.022.1602041
CGCGCAG4750.020.6679461
CGGGCTG5800.019.9488951
CGGGAAG54100.019.701951
CGGGCAA13800.018.0389881
CGGCAAT29550.018.035121
CGCCAAG27000.017.7905641
CGCGAAT14250.017.7153841
TGTCGGC15800.017.49876430
CGGCAAA67650.017.3105771
CGGGAAT22250.016.861131
CGCCAAA38750.016.648651
CGCCAAT26900.016.5532911
CGACAAG32800.016.4618931
CGGCGGC17650.016.45776233
Produced by FastQC (version 0.11.5)