FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6922026
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135940.1963875894138508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA149500.024.93128453
CCGTATC138500.024.5361155
TCGCCGT150700.024.40763752
GTCGCCG174050.024.16944351
GGTCGCC175250.024.06385850
CGTATCA144800.024.02448556
TGGTCGC167000.023.72281349
GCCGTAT164300.023.66533954
AAAGGGG65850.022.85329870
GTATCAT143700.022.23567257
CATTAAA172650.021.8315961
CTCGGTG163800.021.7719144
TCATTAA164050.021.76006360
ATCTCGG167850.021.53848842
GTGGTCG190800.021.40566448
GATCTCG165200.021.22725741
TCTCGGT163050.021.185243
GGTGGTC196300.020.75242847
ATCATTA166600.020.6287459
CGAGATC25600.020.3760765
Produced by FastQC (version 0.11.5)