FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4376292
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94550.2160504829202439No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC21450.033.619095
AAAAGGG126150.029.43506870
CGTATCA195050.028.43938856
CCGTATC193400.028.35629355
CTAGATC20400.028.1423135
CATTAAA212850.028.05060861
TCATTAA206300.027.75370860
CGCCGTA209750.027.61423153
GTATCAT192950.027.33413957
TCGCCGT210500.027.29970652
GTCGCCG232250.027.04865351
GCCGTAT224950.026.82181754
GGTCGCC234450.026.73512650
AAAGGGG51700.026.53581670
TGGTCGC228350.026.25386849
CCGGATC19500.026.20325355
CGCGAAG5950.025.9365061
ATCATTA220000.025.80269659
CTCGGTG206050.025.6642644
TCGCGAG2900.025.3743212
Produced by FastQC (version 0.11.5)