FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6089608
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101690.1669894022735125No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG63520.10430884877975724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGC15950.023.03898833
TGTCGGC17050.021.552630
CGCGCAG3150.021.1520441
CGCGAAT7500.021.0407161
CGCCGTA84750.020.97774353
CGCGAAG8750.020.840331
CCGTATC78750.020.66508355
CGGGAAG57100.020.5739651
CGTATCA82150.020.36362656
TCGCCGT87950.019.9757352
GCCGTAT93400.019.82182354
TCGGCGG18700.019.65090232
GTCGCCG96450.019.48528951
GGTCGCC97150.019.41693950
GTCGGCG18750.019.41184631
CGGCAAG19550.018.8344021
TGGTCGC97700.018.77031349
CTCGGTG88150.018.38202944
CGCGAAA10700.018.0255361
GTATCAT87650.017.88796257
Produced by FastQC (version 0.11.5)