FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4467317
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89390.20009773203916356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG93150.035.01654470
CCGTATC237800.033.92339355
CGAGATC34400.033.7863585
CGTATCA245600.033.62976556
CTAGATC29950.033.546475
CGCCGTA253500.033.49292453
TCGCCGT251600.033.45373552
AAGATCG152950.033.045436
GCCGTAT274750.033.02972854
GTCGCCG290250.032.89367351
GGTCGCC292450.032.81376350
GTATCAT241100.032.3703857
CATTAAA279200.032.3152661
CTCGGTG263100.032.16441344
TCTCGGT262600.032.07905243
ATCTCGG271650.032.05389842
GATCTCG263200.031.96603641
TGGTCGC277100.031.94129449
TCATTAA273050.031.85109960
AGATCTC266050.031.5578340