FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4468255
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85520.19139462720905587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC119200.023.57633855
CGTATCA121750.023.45622856
CATTAAA136350.022.8953261
CGGCAAG13500.022.8553851
TCATTAA132450.022.82962660
GTATCAT119450.022.73592257
CGGCGGC11250.022.70948833
CGCCGTA129950.022.43394953
TGTCGGC11000.022.27113530
TCGCCGT131400.022.13312152
GCCGTAT140300.022.12600354
CGCGAAA7650.021.5415061
GTCGCCG147000.021.5222751
ATCATTA135600.021.31853759
GGTCGCC149600.021.05464650
CGAGATC16350.020.9839045
AAAGGGG37400.020.9610770
TGGTCGC146750.020.67654649
TATCATT134150.020.6619658
CGACCAG3400.020.6248461
Produced by FastQC (version 0.11.5)