FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033053
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49150.24175464191046667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAG802.429897E-630.6890951
CGGCTAG1253.4851837E-928.0586011
CAGATCG10750.027.0298465
CGAGCAG4250.025.5828421
CGGCGGC6100.025.24398633
CCGTATC45600.024.78978555
CGAGATC6600.024.3998895
CGCCGTA48450.024.34283853
CCGGATC4750.024.31394455
CGTATCA46900.024.17726956
TGTCGGC6000.023.91485230
TCGCCGT48550.023.42767552
CGGCAAG5100.023.3821681
CGCCGGA6300.023.33156453
CATTAAA53650.023.15760861
GCCGTAT52900.023.08898554
TCGCCGG5650.022.91861352
GTCGCCG55450.022.84768151
GTCGCCC2004.3655746E-1122.74827450
CTAGATC7250.022.695195
Produced by FastQC (version 0.11.5)