FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6513243
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137700.21141541932336935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA219650.029.5562356
CCGTATC216550.029.54298255
CGCCGTA234200.029.0051453
TCGCCGT232350.028.79927352
AAAAGGG147400.028.53929970
GTCGCCG251150.028.41321651
GCCGTAT250500.028.1935554
GGTCGCC254500.027.99795550
CTCGGTG222500.027.73055844
TGGTCGC251150.027.53531849
GATCTCG218800.027.49570741
TCTCGGT224650.027.3093843
ATCTCGG228150.027.27392242
AGATCTC225850.027.00931740
CGAGATC23650.026.939325
GTATCAT230000.026.6284957
GTGGTCG265300.026.51521548
AAGATCG126200.026.1540366
CAGATCG52000.025.9181355
GGTGGTC271200.025.73189547
Produced by FastQC (version 0.11.5)