FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_vk5-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_vk5-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5110603
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122690.2400695182153652No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG133850.030.85329251
GGTCGCC133550.030.81777650
TCGCCGT98700.030.56525252
TGGTCGC114150.029.83147849
TCGCCGG37250.029.59525952
CGAGATC23850.029.5023125
ATCTCGG124000.029.4374742
CTCGGTG126200.029.36800844
CGCCGTA95850.029.35633353
TCTCGGT120200.029.17434743
CTAGATC25700.029.1493595
GATCTCG124050.029.1152741
GTGGTCG141600.029.04106148
CCGTATC90850.029.00734155
CGTATCA102750.028.91770256
GGGTCGC34250.028.917749
AAGATCG127450.028.891436
CGCCGGA39800.028.84221653
CCGGATC35000.028.49801855
CCGTGTC12100.028.34513955
Produced by FastQC (version 0.11.5)