FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33612477
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4994961.4860434117961614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC688350.029.99396355
CGTATCA711150.029.65322956
CGCCGTA744550.029.44212753
TCGCCGT739050.029.23959552
GCCGTAT811250.028.49804554
CTCGGTG714350.027.74270844
TCTCGGT716050.027.59490643
GATCTCG703050.027.43078241
GTCGCCG867200.027.19710451
CGAGATC92550.027.1832965
AGATCTC728300.026.68616740
TGGTCGC842350.026.5668449
GTATCAT748050.026.52947657
GGTCGCC902100.026.08440850
GTGGTCG904100.025.3102148
TCATTAA852700.025.16259460
TCGGTGG900250.025.05249445
TAGATCT823650.024.84990539
CAGATCG178400.024.8157715
AAAAGGG544300.024.75538370
Produced by FastQC (version 0.11.5)