FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670786
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG170900.029.53139764
GCTTGAA177250.029.22377266
TGCTTGA178800.028.32446965
ACACTCT29800.028.0801031
GCCGTCT206750.026.7806956
TCTGCTT197050.025.98535563
CCGTCTT216000.025.76345857
TGCCGTC226250.025.58631355
CTTGAAA211750.025.0904967
TATGCCG235400.024.94860553
ATGCCGT234850.024.91761854
GAGATCG74950.024.56656
GTATGCC245950.024.10612552
CGTATGC245550.024.07412351
TCGTATG245550.023.77480150
GTCTTCT224050.023.74430559
CTCGTAT234500.023.31304249
GATCTCG220500.023.22183446
CGTCTTC236600.023.16529558
AAGATCG116900.023.087086
Produced by FastQC (version 0.11.5)