FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9474629
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT49650.034.4882661
CACTCTT97900.019.6725981
AGTGGCG12000.019.54117439
GCCCACA23750.017.103151
GCGGTAG14350.016.3410543
CGCGGAC5500.015.908689546
GGCGGTA14600.015.58179842
CGGTAGT16950.014.45391344
CCGAGCC7450.014.09360452
ACCGAGC7500.013.99964751
CCGAGTG7450.013.62381761
ACAGGCT17350.013.5188624
GCGGACA6250.013.4396647
AGGCTTG19300.013.0599046
GGCGATT16600.012.65028454
TGGCGGT18050.012.603559541
GAGCTGC18750.012.3196932
CCAGAGG21050.012.13746122
AACAGGC18550.012.0781623
CTTACGC16100.011.9591876
Produced by FastQC (version 0.11.5)