FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6420848
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT27700.036.5322381
GCCCACA17000.028.4242081
CGAGCCT4500.023.33282753
ACCGAGC4200.022.49951251
AGTGGCG6250.022.39951539
GCGGTAG6600.021.2116643
GCCACAC15350.019.6177081
CCGAGCC5450.019.26563852
CACTCTT66300.018.3262731
TGCGCGA2802.0918378E-1017.4996212
GCGCGAT2852.6739144E-1017.1926113
GTGCGCG2852.6739144E-1017.1926111
GCGTGAA7750.017.16091766
GGCGGTA7800.017.0509142
CGCGGAC4250.016.4702346
GAGTGGC4300.016.27871763
AGTGCGC3451.4551915E-1116.23153310
AGGCTTG10000.016.1030376
CGAGTGG4150.016.02374862
TACGCGC2201.3975878E-615.90874722
Produced by FastQC (version 0.11.5)