FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7344079
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT33250.030.1191621
CGCTTGC10100.020.79162423
GCGCGAT4050.019.01192913
GTGGCGG10150.018.96510140
AGTGGCG9600.018.95791839
TGCGCGA4150.018.5538112
GCGCTTG11750.018.4676822
CGCGGAC4550.018.46113446
CCGAGCC5800.018.10305252
GCGGTAG10250.017.75570943
CGCGATC4800.017.49961714
GGCGGTA10400.017.16308642
GCCCACA18750.016.0607451
CACTCTT79600.015.8804441
ACCGAGC6700.015.67129951
GAGCTGC11900.015.29378232
CGGTAGT11900.015.29378244
CGAGTGG5200.014.80736762
GCGGAGC3604.674803E-1014.58301423
GGCGATT10000.014.34968654
Produced by FastQC (version 0.11.5)