FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6824642
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGC4150.022.77073714
TGCGCGG4050.022.46879212
ACACTCT29750.018.8291741
GCGGGCA4900.018.57114415
ACGAGTG10650.018.40347538
GCCCACA20050.018.1600591
CACGAGT11650.017.72505237
ACGGAGT11300.017.3474086
CGGAGTC12200.016.6392147
CGAGTGA12300.016.21926539
CTGCGCG5400.016.20345711
GAGTCAC14000.015.49976359
CCGAGCC6400.015.31226552
CGGACAG5050.015.24729248
CCACGAG14050.015.195497536
GAGTGAG13350.014.68142340
ACTCGGC6450.014.65093956
GTGGCGG9700.014.4327740
AGAGCGC8250.014.42402258
GTGAGGT13600.014.41154542
Produced by FastQC (version 0.11.5)