FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9726109
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT58700.028.0314431
AGTGGCG16050.020.2801339
GCCCACA23650.019.3921431
GTGGCGG18050.019.3903540
GGCGGTA17350.018.76058442
CCGAGCC9100.017.69209752
GCGGTAG18300.017.2129143
CACTCTT111950.016.480571
TGGCGGT20950.015.53680741
GTGCGCG4351.8189894E-1214.48258611
GGCGATT22000.014.477154
ACCGAGC10500.014.33316251
CGAGCCT11250.014.31094153
GTTGCCG22500.013.68872569
CGGTAGT23700.013.29098144
GAGCTGC23300.013.21872632
AGGCTTG26000.013.1935756
ACAGGCT26400.013.1262624
CCAGAGG26400.012.992269522
GAGCGCA12350.012.46948759
Produced by FastQC (version 0.11.5)