FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6922026
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT37800.030.7534871
GCCCACA30400.027.2974971
GAGATCG82550.022.8563466
GGGTGGA16700.021.58641868
ATCGGGG103300.020.32875449
AATCGGG118100.020.15203548
TGGCGGG23900.020.06238457
GGGGGGA17900.019.7482368
GCCACAC30000.018.9078371
GAATCGG131900.018.68046647
TCGGGGT47600.018.52906250
CGGGGTT39950.018.22243551
GGAATCG188250.018.01559646
GCGTGGG10150.017.93069662
TCGGGGG59750.017.92434950
CGGAATC251000.017.86221345
GTTTCGG317450.017.79459241
CACTCTT78700.017.7074681
CGGGGGT42150.017.60346451
TCGGAAT290200.017.58409144
Produced by FastQC (version 0.11.5)